URLhttps://www.bco-dmo.org/dataset/565366
Download URLhttps://www.bco-dmo.org/dataset/565366/data/download
Media Typetext/tab-separated-values
CreatedSeptember 2, 2015
ModifiedFebruary 16, 2018
StatePreliminary and in progress
Brief DescriptionNorth Pond CORKs dissolved ion concentrations from samples collected using the MVBS system during ROV Jason-II dives

Acquisition Description

Standard Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) run on 0.2um filtered, acidified, subsamples from fluid sampling from IODP CORK Observatories.

Details for ROV sampling instrumentation are provided in Cowen, J.P., Copson, D., Jolly, J., Hsieh, C.-C., Matsumoto, R., Glazer, B.T. et al. (2012) Advanced instrument system for real-time and time-series microbial geochemical sampling of the deep (basaltic) crustal biosphere. Deep-Sea Research I, 61: 43-56.

Processing Description

BCO-DMO Processing:
- Added conventional header with dataset name, PI name, version date
- Modified column headers to conform to BCO-DMO naming conventions.

Instruments

GeoMICROBE [GeoMICROBE]
Details
Instance Description (GeoMICROBE)

Autonomous sled with sensors and fluid sampling system.

Integrated Ocean Drilling Program borehole CORK (Circulation Obviation Retrofit Kit) observatories provide long-term access to hydrothermal fluids circulating within the basaltic crust (basement), providing invaluable opportunities to study the deep biosphere. We describe the design and application parameters of the GeoMICROBE instrumented sled, an autonomous sensor and fluid sampling system. The GeoMICROBE system couples with CORK fluid delivery lines to draw large volumes of fluids from crustal aquifers to the seafloor. These fluids pass a series of in-line sensors and an in situ filtration and collection system. GeoMICROBE’s major components include a primary valve manifold system, a positive displacement primary pump, sensors (e.g., fluid flow rate, temperature, dissolved O2, electrochemistry-voltammetry analyzer), a 48-port in situ filtration and fluid collection system, computerized controller, seven 24 V-40 A batteries and wet-mateable (ODI) communications with submersibles. This constantly evolving system has been successfully connected to IODP Hole 1301A on the eastern flank of the Juan de Fuca Ridge. 

Reference: Cowen, J.P., Copson, D., Jolly, J., Hsieh, C.-C., Matsumoto, R., Glazer, B.T. et al. (2012) Advanced instrument system for real-time and time-series microbial geochemical sampling of the deep (basaltic) crustal biosphere., Deep-Sea Research I, 61: 43-56 doi:10.1016/j.dsr.2011.11.004

Parameters

sample [sample]
Details
sample

sample id; J2-### refers to ROV Jason-2 dive number

unique sample identification or number; any combination of alpha numeric characters; precise definition is file dependent

site [site]
Details
site

sample site: borehole and relative depth

Sampling site identification.
B [B]
Details
B
concentration of dissolved Boron

B (Boron) concentration. May be reported in parts per million, nanomoles/Liter, or other units. Refer to dataset metadata for units.

Ca [Ca]
Details
Ca
concentration of dissolved Calcium

Ca (Calcium) concentration. May be reported in parts per million, nanomoles/Liter, or other units. Refer to dataset metadata for units.

K [K]
Details
K
concentration of dissolved Potassium

K (potassium) concentration. May be reported in parts per million, nanomoles/Liter, or other units. Refer to dataset metadata for units.

Mg [Mg]
Details
Mg
concentration of dissolved Magnesium

Mg (magnesium) concentration. May be reported in parts per million, nanomoles/Liter, or other units. Refer to dataset metadata for units.

Sr [Sr]
Details
Sr
concentration of dissolved Strontium

Sr (Strontium) concentration. May be reported in parts per million, nanomoles/Liter, or other units. Refer to dataset metadata for units.

Fe [Fe]
Details
Fe
concentration of dissolved Iron
Fe (Iron) concentration. May be reported in parts per million, nanomoles/Liter, or other units. Refer to dataset metadata for units.
Mn [Mn]
Details
Mn
concentration of dissolved Manganese

Mn (Manganese) concentration. May be reported in parts per million, nanomoles/Liter, or other units. Refer to dataset metadata for units.

Dataset Maintainers

NameAffiliationContact
Brian T. GlazerUniversity of Hawaii at Manoa (SOEST)
Julie A. HuberMarine Biological Laboratory (MBL)
Peter R. GirguisHarvard University
Nancy CopleyWoods Hole Oceanographic Institution (WHOI BCO-DMO)

BCO-DMO Project Info

Project TitleCollaborative Research: Characterization of Microbial Transformations in Basement Fluids, from Genes to Geochemical Cycling
AcronymNorth Pond Microbes
URLhttps://www.bco-dmo.org/project/554914
CreatedApril 3, 2015
ModifiedAugust 29, 2019
Project Description

Description from NSF award abstract:
Current estimates suggest that the volume of ocean crust capable of sustaining life is comparable in magnitude to that of the oceans. To date, there is little understanding of the composition or functional capacity of microbial communities in the sub-seafloor, or their influence on the chemistry of the oceans and subsequent consequences for global biogeochemical cycles. This project focuses on understanding the relationship between microbial communities and fluid chemistry in young crustal fluids that are responsible for the transport of energy, nutrients, and organisms in the crust. Specifically, the PIs will couple microbial activity measurements, including autotrophic carbon, nitrogen and sulfur metabolisms as well as mineral oxide reduction, with quantitative assessments of functional gene expression and geochemical transformations in basement fluids. Through a comprehensive suite of in situ and shipboard analyses, this research will yield cross-disciplinary advances in our understanding of the microbial ecology and geochemistry of the sub-seafloor biosphere. The focus of the effort is at North Pond, an isolated sediment pond located on ridge flank oceanic crust 7-8 million years old on the western side of the Mid-Atlantic Ridge. North Pond is currently the target for drilling on IODP expedition 336, during which it will be instrumented with three sub-seafloor basement observatories.

The project will leverage this opportunity for targeted and distinct sampling at North Pond on two German-US research cruises to accomplish three main objectives:

1. to determine if different basement fluid horizons across North Pond host distinct microbial communities and chemical milieus and the degree to which they change over a two-year post-drilling period.

2. to quantify the extent of autotrophic metabolism via microbially-mediated transformations in carbon, nitrogen, and sulfur species in basement fluids at North Pond.

3. to determine the extent of suspended particulate mineral oxides in basement fluids at North Pond and to characterize their role as oxidants for fluid-hosted microbial communities.

Specific outcomes include quantitative assessments of microbial activity and gene expression as well as geochemical transformations. The program builds on the integrative research goals for North Pond and will provide important data for guiding the development of that and future deep biosphere research programs. Results will increase understanding of microbial life and chemistry in young oceanic crust as well as provide new insights into controls on the distribution and activity of marine microbial communities throughout the worlds oceans.

There are no data about microbial communities in ubiquitous cold, oceanic crust, the emphasis of the proposed work. This is an interdisciplinary project at the interface of microbial ecology, chemistry, and deep-sea oceanography with direct links to international and national research and educational organizations.

Project Maintainers
NameAffiliationRoleContact
Julie A. HuberMarine Biological Laboratory (MBL)Lead Principal Investigator
Peter R. GirguisHarvard UniversityPrincipal Investigator
Brian T. GlazerUniversity of Hawaii at Manoa (SOEST)Principal Investigator
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