URLhttps://www.bco-dmo.org/dataset/630335
Download URLhttps://www.bco-dmo.org/dataset/630335/data/download
Media Typetext/tab-separated-values
CreatedDecember 30, 2015
ModifiedAugust 19, 2016
Brief DescriptionParticulate organic carbon from the formation fluids recovered from the CORKs installed at the North Pond in 2012.

Acquisition Description

Carbon and nitrogen concentrations of particulate organic matter were measured by the Biogeochemical Stable Isotope Facility at School of Ocean and Earth Science and Technology, University of Hawaii. A few drops of sulfurous acid (6-9 % of H2SO3) were added to wet the filter and to remove inorganic carbon from the filter. The acidified filters were transferred to a 60 degree C oven for 24 hours. The dried filters were then transferred into a tin capsule before placing within a high temperature combustion CN elemental analyzer (Costech, ECS 4010) connected in-line with a Mass Spectrometer (ThermoFinnigan Delta XP interfaced with a ConFloIV) for analysis of their organic carbon and nitrogen isotopic compositions. The detection limit for carbon and nitrogen content is 10 ug-C and 0.3 ug-N, respectively. The limit for reliable carbon and nitrogen isotopic determination is 10 ug-C and 10 ug-N, respectively.

Processing Description

BCO-DMO Processing:
- Modified parameter names to conform with BCO-DMO naming conventions;
- Replaced "n.a." with "nd" to indicate "no data";
- Replaced "u.d." with "bel_det" to indiacte "below detection";
- Replaced commas with semi-colons;
- Replaced spaces with underscores;
- Added cruise id numbers.

Instruments

Costech, ECS 4010 [Elemental Analyzer]
Details
Instance Description (Costech, ECS 4010)

The dried filters were then transferred into a tin capsule before placing within a high temperature combustion CN elemental analyzer (Costech, ECS 4010) connected in-line with a Mass Spectrometer (ThermoFinnigan Delta XP interfaced with a ConFloIV) for analysis of their organic carbon and nitrogen isotopic compositions.

Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material.

ThermoFinnigan Delta XP [Mass Spectrometer]
Details
Instance Description (ThermoFinnigan Delta XP)

The dried filters were then transferred into a tin capsule before placing within a high temperature combustion CN elemental analyzer (Costech, ECS 4010) connected in-line with a Mass Spectrometer (ThermoFinnigan Delta XP interfaced with a ConFloIV) for analysis of their organic carbon and nitrogen isotopic compositions.

General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components.

Parameters

site [site]
Details
site

Sampling site name. The nomenclature refers to the IODP hole and formation horizon. For example, U1383C-shallow means the fluids came from IODP CORK drillhole 1383C in the shallowest accessible porewater horizon.

Sampling site identification.
cruise_id [cruise_id]
Details
cruise_id
Cruise identifier.
cruise designation; name
dive_num [unknown]
Details
dive_num
Dive number.
association with a community-wide standard parameter is not yet defined
method [unknown]
Details
method
Sampling method.
association with a community-wide standard parameter is not yet defined
PN_ug [unknown]
Details
PN_ug
Particulate nitrogen.
association with a community-wide standard parameter is not yet defined
del15N [unknown]
Details
del15N

delta 15N

association with a community-wide standard parameter is not yet defined
OC [unknown]
Details
OC
Organic Carbon (?)
association with a community-wide standard parameter is not yet defined
del13C [delta13C]
Details
del13C

delta 13C

Carbon 13 to Carbon 12 ratio of TCO2; CAUTION file dependent definition changes slightly between EqPac and Southern basin studies
vol_filt [vol_filt]
Details
vol_filt
Volume filtered.

volume of water filtered; in this form generally applies to the volume of water filtered during plankton tows; however; this parameter name is also used for a variety of non plankton sampling and the units can vary, e.g. US JGOFS EqPac Bishop MULVFS sampler vol_filt is reported in liters.

POC [POC]
Details
POC
Particulate organic carbon.

Particulate Organic Carbon. Units may be micrograms/Liter, milligrams/Liter or milligrams/meter^3

PN_umolL [unknown]
Details
PN_umolL
Particulate nitrogen.
association with a community-wide standard parameter is not yet defined
C_to_N [C_to_N]
Details
C_to_N
Molar ratio of carbon to nitrogen.
Carbon to Nitrogen ratio; CAUTION; file dependent
fraction_C [unknown]
Details
fraction_C
1/C
association with a community-wide standard parameter is not yet defined

Dataset Maintainers

NameAffiliationContact
Peter R. GirguisHarvard University
Julie A. HuberMarine Biological Laboratory (MBL)
Brian T. GlazerUniversity of Hawaii at Manoa (SOEST)
Beate KraftHarvard University
Shannon RauchWoods Hole Oceanographic Institution (WHOI BCO-DMO)

BCO-DMO Project Info

Project TitleCollaborative Research: Characterization of Microbial Transformations in Basement Fluids, from Genes to Geochemical Cycling
AcronymNorth Pond Microbes
URLhttps://www.bco-dmo.org/project/554914
CreatedApril 3, 2015
ModifiedAugust 29, 2019
Project Description

Description from NSF award abstract:
Current estimates suggest that the volume of ocean crust capable of sustaining life is comparable in magnitude to that of the oceans. To date, there is little understanding of the composition or functional capacity of microbial communities in the sub-seafloor, or their influence on the chemistry of the oceans and subsequent consequences for global biogeochemical cycles. This project focuses on understanding the relationship between microbial communities and fluid chemistry in young crustal fluids that are responsible for the transport of energy, nutrients, and organisms in the crust. Specifically, the PIs will couple microbial activity measurements, including autotrophic carbon, nitrogen and sulfur metabolisms as well as mineral oxide reduction, with quantitative assessments of functional gene expression and geochemical transformations in basement fluids. Through a comprehensive suite of in situ and shipboard analyses, this research will yield cross-disciplinary advances in our understanding of the microbial ecology and geochemistry of the sub-seafloor biosphere. The focus of the effort is at North Pond, an isolated sediment pond located on ridge flank oceanic crust 7-8 million years old on the western side of the Mid-Atlantic Ridge. North Pond is currently the target for drilling on IODP expedition 336, during which it will be instrumented with three sub-seafloor basement observatories.

The project will leverage this opportunity for targeted and distinct sampling at North Pond on two German-US research cruises to accomplish three main objectives:

1. to determine if different basement fluid horizons across North Pond host distinct microbial communities and chemical milieus and the degree to which they change over a two-year post-drilling period.

2. to quantify the extent of autotrophic metabolism via microbially-mediated transformations in carbon, nitrogen, and sulfur species in basement fluids at North Pond.

3. to determine the extent of suspended particulate mineral oxides in basement fluids at North Pond and to characterize their role as oxidants for fluid-hosted microbial communities.

Specific outcomes include quantitative assessments of microbial activity and gene expression as well as geochemical transformations. The program builds on the integrative research goals for North Pond and will provide important data for guiding the development of that and future deep biosphere research programs. Results will increase understanding of microbial life and chemistry in young oceanic crust as well as provide new insights into controls on the distribution and activity of marine microbial communities throughout the worlds oceans.

There are no data about microbial communities in ubiquitous cold, oceanic crust, the emphasis of the proposed work. This is an interdisciplinary project at the interface of microbial ecology, chemistry, and deep-sea oceanography with direct links to international and national research and educational organizations.

Project Maintainers
NameAffiliationRoleContact
Julie A. HuberMarine Biological Laboratory (MBL)Lead Principal Investigator
Peter R. GirguisHarvard UniversityPrincipal Investigator
Brian T. GlazerUniversity of Hawaii at Manoa (SOEST)Principal Investigator
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