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Awards > Research Grants
Metagenomics, metatranscriptomics, and single-cell genomics of microbial communities inhabiting Juan de Fuca Ridge flank borehole fluids
PI: Michael S. Rappé (University of Hawaii)
Co-I: Sean P. Jungbluth (University of Hawaii)
Current Placement: Postdoctoral Research Scholar, USC, 2015-
Amount: $49,204.00
Award Dates: September 15, 2011 — September 14, 2013

Abstract

We are investigating the deep biosphere of the subseafloor basaltic crust on the eastern flank of the Juan de Fuca Ridge by accessing pristine crustal fluids via Circulation Obviation Retrofit Kit (CORK) sampling and instrumentation platforms. Second-generation CORKs installed within Integrated Ocean Drilling Program boreholes provide unprecedented opportunities to study the microbial ecology and biogeochemistry of fluids circulating in the sediment-covered oceanic basement. In the summers of 2008-2011, a unique large-volume sampling system was used to collect some of the only large volume pristine basement fluid samples available for microbiological studies to date, from a 3.5 Myr-old basalt-hosted basement aquifer that is covered by 260m of sediment. On an annual scale, the microbial community appears to be temporally dynamic; however, some common community members are shared between sample years. We will obtain nucleic acid sequence data from the metagenome of two deep subsurface microbial community samples (one each from field seasons 2010 and 2011) and the metatranscriptome of one corresponding sample, as well as amplify the genomes of a subset of single cells. Due to the well-known difficulties in obtaining pristine sub-seafloor ocean crustal fluids, these samples offer an unmatched opportunity to investigate the community genomics of cells inhabiting the deep subseafloor biosphere.

Related Items

Publications
Publications > Proceedings
Published: September 5, 2011
Proceedings of the IODP
Data report: microbial diversity in sediment near Grizzly Bare Seamount in Holes U1363B and U1363G
Authors: Sean P. Jungbluth, L. G. H. Johnson, James P. Cowen, Michael S. Rappé
C-DEBI Contribution Number: 181
Publications > Journal Article
Published: March 25, 2014
Frontiers in Microbiology
Phylogenetic diversity of microorganisms in subseafloor crustal fluids from Holes 1025C and 1026B along the Juan de Fuca Ridge flank
Authors: Sean P. Jungbluth, Huei-Ting Lin, James P. Cowen, Brian T. Glazer, Michael S. Rappé
C-DEBI Contribution Number: 244
Publications > Journal Article
Published: January 14, 2015
Frontiers in Microbiology
Activity and phylogenetic diversity of sulfate-reducing microorganisms in low-temperature subsurface fluids within the upper oceanic crust
Authors: Alberto Robador, Sean P. Jungbluth, Douglas E. LaRowe, Robert M. Bowers, Michael S. Rappé, Jan P. Amend, James P. Cowen
C-DEBI Contribution Number: 249
Publications > Journal Article
Published: February 12, 2016
The ISME Journal
Novel microbial assemblages inhabiting crustal fluids within mid-ocean ridge flank subsurface basalt
Authors: Sean P. Jungbluth, Robert M. Bowers, Huei-Ting Lin, James P. Cowen, Michael S. Rappé
C-DEBI Contribution Number: 289
Project Data
Project Data
Last Modified: December 15, 2016
Microbiology and biogeochemistry of Juan de Fuca Ridge flank borehole fluids
Project Maintainers: Michael S. Rappé

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