CENTER for DARK ENERGY BIOSPHERE INVESTIGATIONS
Menu
  • About
    • Introduction
    • People
    • Spotlight
    • Ethics Policy
    • Data Access & Policy
    • Executive Documents
    • C-DEBI Branded Resources
    • Support C-DEBI
    • Contact Us
  • Research
    • Overview
    • Research Themes
    • Field Sites
    • Facilities, Equipment & Technology
    • Research Support
      • Research Grants
      • Graduate Fellowships
      • Postdoctoral Fellowships
      • Research Exchanges
      • Community Workshops
      • Funded Projects
  • Education
    • Overview
    • For Teachers
    • For High School Students
    • For Undergraduates
      • Overview
      • GEM Summer Course
      • C4 Research Experience
      • CC-RISE Research Internship
      • GGURE Research Experience
      • Partnering Organizations
    • For Graduates & Postdocs
    • For Everyone
    • Education Grants
  • Resources
    • Peer-Reviewed Publications
    • Project Data
    • Protocols
    • Subseafloor Cultures Database
    • Videos
    • Mailing List & Newsletter
    • Meetings & Workshops
    • Networked Speaker Series
    • Professional Development Webinars
    • How to Get Deep Biosphere Samples

Person: Eoin L. Brodie

Type
All
Awards
Publications
Order
Date Desc
Date Asc
Title Asc
Title Desc
Publications > Journal Article
Published: November 1, 2013
Marine and Petroleum Geology
Microbial distributions detected by an oligonucleotide microarray across geochemical zones associated with methane in marine sediments from the Ulleung Basin
Authors: Brandon R. Briggs, Michael F. Graw, Eoin L. Brodie, Jang-Jun Bahk, Sung-Han Kim, Jung-Ho Hyun, Ji-Hoon Kim, Marta Torres, Frederick S. Colwell
C-DEBI Contribution Number: 152
Awards > RCN Research Exchange Grants
Award Dates: June 27, 2011 — July 1, 2011
The Effect of Methane on Microbial Community Distributions in the Ulleung Basin
PI: Brandon R. Briggs (Oregon State University)
Current Placement: Assistant Professor, University of Alaska Anchorage
Advisor: Frederick S. Colwell (Oregon State University)
Host: Eoin L. Brodie (Lawrence Berkeley National Laboratory)
Publications > Journal Article
Marine and Petroleum Geology
Microbial distributions detected by an oligonucleotide microarray across geochemical zones associated with methane in marine sediments from the Ulleung Basin
Authors: Brandon R. Briggs, Michael F. Graw, Eoin L. Brodie, Jang-Jun Bahk, Sung-Han Kim, Jung-Ho Hyun, Ji-Hoon Kim, Marta Torres, Frederick S. Colwell
Published: November 1, 2013
C-DEBI Contribution Number: 152

Abstract

The biogeochemical processes that occur in marine sediments on continental margins are complex; however, from one perspective they can be considered with respect to three geochemical zones based on the presence and form of methane: sulfate–methane transition (SMTZ), gas hydrate stability zone (GHSZ), and free gas zone (FGZ). These geochemical zones may harbor distinct microbial communities that are important in biogeochemical carbon cycles. The objective of this study was to describe the microbial communities in sediments from the SMTZ, GHSZ, and FGZ using molecular ecology methods (i.e. PhyloChip microarray analysis and terminal restriction fragment length polymorphism (T-RFLP)) and examining the results in the context of non-biological parameters in the sediments. Non-metric multidimensional scaling and multi-response permutation procedures were used to determine whether microbial community compositions were significantly different in the three geochemical zones and to correlate samples with abiotic characteristics of the sediments. This analysis indicated that microbial communities from all three zones were distinct from one another and that variables such as sulfate concentration, hydrate saturation of the nearest gas hydrate layer, and depth (or unmeasured variables associated with depth e.g. temperature, pressure) were correlated to differences between the three zones. The archaeal anaerobic methanotrophs typically attributed to performing anaerobic oxidation of methane were not detected in the SMTZ; however, the marine benthic group-B, which is often found in SMTZ, was detected. Within the GHSZ, samples that were typically closer to layers that contained higher hydrate saturation had indicator sequences related to Vibrio-type taxa. These results suggest that the biogeographic patterns of microbial communities in marine sediments are distinct based on geochemical zones defined by methane.

Source: http://dx.doi.org/10.1016/j.marpetgeo.2013.02.015

Related Items

Awards
Awards > RCN Research Exchange Grants
Award Dates: June 27, 2011 — July 1, 2011
The Effect of Methane on Microbial Community Distributions in the Ulleung Basin
PI: Brandon R. Briggs (Oregon State University)
Current Placement: Assistant Professor, University of Alaska Anchorage
Advisor: Frederick S. Colwell (Oregon State University)
Host: Eoin L. Brodie (Lawrence Berkeley National Laboratory)
Awards > RCN Research Exchange Grants
The Effect of Methane on Microbial Community Distributions in the Ulleung Basin
PI: Brandon R. Briggs (Oregon State University)
Current Placement: Assistant Professor, University of Alaska Anchorage
Advisor: Frederick S. Colwell (Oregon State University)
Host: Eoin L. Brodie (Lawrence Berkeley National Laboratory)
Amount: $4,538.54
Award Dates: June 27, 2011 — July 1, 2011

The DEBI/C-DEBI research exchange gave me the opportunity to travel to Lawrence Berkeley National Laboratory (LBNL) to work with Dr. Eoin Brodie. While at LBNL, I was able to learn and adopt a new microarray technology for subseafloor microbial ecology investigations. The PhyloChip is a microarray that contains probes for Bacterial and Archaeal 16s rRNA genes and uses parallel hybridization to minimizes the influence of dominant organisms; therefore, it is highly sensitive to rare microbes. In addition, to this data the research exchange provided necessary funding for me to finish my dissertation. The research exchange funds allowed two sites in the Ulleung Basin to be fully analyzed and incorporated into my dissertation “Geomicrobiology of sediment containing methane”. This collaboration also introduced me to how research is conducted at a national laboratory. This information is valuable in deciding my future career path. Whatever that path may be the collaboration and techniques that I learned will continue beyond graduate school, as we are planning future studies using the PhyloChip. For more on this method, see Briggs, Pohlman, Torres, Reidel, Brodie and Colwell’s 2011 AEM paper Macroscopic biofilms in fracture-dominated sediment that anaerobically oxidize methane.

Related Items

Publications
Publications > Journal Article
Published: November 1, 2013
Marine and Petroleum Geology
Microbial distributions detected by an oligonucleotide microarray across geochemical zones associated with methane in marine sediments from the Ulleung Basin
Authors: Brandon R. Briggs, Michael F. Graw, Eoin L. Brodie, Jang-Jun Bahk, Sung-Han Kim, Jung-Ho Hyun, Ji-Hoon Kim, Marta Torres, Frederick S. Colwell
C-DEBI Contribution Number: 152
Project Data
Project Data
Last Modified: June 14, 2018
The Effect of Methane on Microbial Community Distributions in the Ulleung Basin
Project Maintainers: Brandon R. Briggs

About C-DEBI  |  Our Research  |  Education & Outreach  |  Resources  |
Contact Us  |  Join Our Mailing List  |  Find Us On Facebook  |  Follow us on Twitter
© 2009-2019 Center for Dark Energy Biosphere Investigations (C-DEBI)

SUPPORT C-DEBI TODAY |  National Science Foundation