The Deep Life Community (DLC) within the Sloan Foundation supported Deep Carbon Observatory realizes that the majority of deep microbial life has been resistant to cultivation in the laboratory, which complicates the characterization of physiological characteristics of deep community members. However, recent studies using bioreactor-cultivation techniques, under high pressure and/or temperature, have resulted in successful enrichment of previously uncultivable archaeal and bacterial components that mediate biogeochemical carbon cycling in deep subsurface (1-7). In order to maintain and strengthen cultivation strategies in future deep life missions, the DLC will support early-carrier researchers to visit some key laboratories (Inagaki – Kochi, Japan, Bartlett – La Jolla, USA, and others) to learn and practice newly developed cultivation and cultivation-dependent molecular/biogeochemical techniques using samples from the DLC’s field missions. Financial support includes $5,400 per person for travel and lodging costs and host lab research supply reimbursement. Interested applicants should send their cv, a brief one page statement of their cultivation plans, and a letter of support from their intended host to Fumio Inagaki (firstname.lastname@example.org ) and Douglas Bartlett (email@example.com).
The Deep Carbon Observatory, in collaboration with the Department of Earth Sciences of Sapienza University (Rome), is hosting its third Early Career Scientist Workshop in Nicolosi (Etna), Italy, 28 August-2 September 2017. This workshop will bring together the next generation of researchers active in deep carbon studies from around the world. Building on the success of the first and second DCO Early Career Scientist Workshops, this third workshop (~50 scientists) of early career researchers will continue to foster collaboration and community within the ever expanding DCO Science Network. The workshop is funded by the Alfred P. Sloan Foundation and aims to financially support as many participants as possible. There is no registration fee for this workshop (accommodation and meals will be provided). Successful applicants will be eligible for up to 100% reimbursement of travel costs. Senior graduate students, postdoctoral researchers, fellows, and newly appointed assistant professors, are encouraged to apply. The application window is open through April 14, 2017.
Since 2011, the Deep Carbon Observatory’s (DCO) Deep Life Community has sponsored the Census of Deep Life (CoDL) that has supported surveys of the diversity of microbes present in several deep continental and subseafloor environments. The first surveys (2011-2012) were conducted using 454 pyrosequencing and subsequently (2013) Illumina sequencing strategies were adopted. Through this initiative, the Deep Life Community has allowed the characterization of diversity of subsurface microbial communities at numerous sites worldwide including the subseafloor and deep continental locations from a range of geologic settings (e.g., large igneous provinces, subglacial lakes, methane hydrate-rich sediments, cratons). The Illumina platform provides increased numbers of reads for more samples at reduced cost. For DNA samples submitted to the CoDL for sequencing, proponents have the option of obtaining 400-450 nt sequences that span the V4V5 region of Bacterial and Archaeal rRNA coding regions or a greater number of reads for V6 regions that through complete overlap of forward and reverse reads allows detection of lower abundance taxa with reduced stochastic error rates. Shotgun metagenomic DNA sequencing for key samples can also be performed. This call for proposals aims to support sequencing that represents expanded analyses from ongoing Deep Life Community projects or projects that represent sites and investigators new to the DCO’s Deep Life Community. Proposal deadline: April 30, 2017.
The Deep Energy Community (DEC) of the Deep Carbon Observatory invites proposals for short- term funding of projects and/or activities aimed at addressing the DEC’s decadal goals and/or strengthening the international DEC community and its abilities to generate funding for new and ongoing initiatives. The DEC is dedicated to quantifying the environmental conditions and processes from the molecular to the global scale that control the origins, forms, quantities and movements of reduced carbon compounds derived from deep carbon through deep geologic time. The DEC has identified a number of guiding questions and the DEC Steering Committee encourages submission of ideas for modest short-term support that will address these and other relevant / meritorious efforts with high potential to attract new funding. Examples of supported activities include 1) laboratory research, 2) travel to field sites to collect samples of key importance, 3) support of working groups and workshops to synthesize data for publication of Deep Energy research, and/or to develop interdisciplinary collaborations, 4) travel to work with collaborators on the preparation of new proposals, or 5) other activities that would advance Deep Energy Goals. Application deadline: November 20, 2016.