{"id":7775,"date":"2016-06-14T23:02:46","date_gmt":"2016-06-14T23:02:46","guid":{"rendered":"https:\/\/www.darkenergybiosphere.org\/?post_type=publication&p=7764"},"modified":"2016-10-14T19:06:49","modified_gmt":"2016-10-14T19:06:49","slug":"structural-iron-ii-of-basaltic-glass-as-an-energy-source-for-zetaproteobacteria-in-an-abyssal-plain-environment-off-the-mid-atlantic-ridge","status":"publish","type":"publication","link":"https:\/\/www.darkenergybiosphere.org\/publication\/structural-iron-ii-of-basaltic-glass-as-an-energy-source-for-zetaproteobacteria-in-an-abyssal-plain-environment-off-the-mid-atlantic-ridge\/","title":{"rendered":"Structural Iron (II) of Basaltic Glass as an Energy Source for Zetaproteobacteria in an Abyssal Plain Environment, Off the Mid Atlantic Ridge"},"content":{"rendered":"

To explore the capability of basaltic glass to support the growth of chemosynthetic microorganisms, complementary in situ<\/em> and in vitro<\/em> colonization experiments were performed. Microbial colonizers containing synthetic tholeitic basaltic glasses, either enriched in reduced or oxidized iron, were deployed off-axis from the Mid Atlantic Ridge on surface sediments of the abyssal plain (35\u00b0N; 29\u00b0W). In situ<\/em> microbial colonization was assessed by sequencing of the 16S rRNA gene and basaltic glass alteration was characterized using Scanning Electron Microscopy, micro-X-ray Absorption Near Edge Structure at the Fe-K-edge and Raman microspectroscopy. The colonized surface of the reduced basaltic glass was covered by a rind of alteration made of iron-oxides trapped in a palagonite-like structure with thicknesses up to 150 \u03bcm. The relative abundance of the associated microbial community was dominated (39% of all reads) by a single operational taxonomic unit (OTU) that shared 92% identity with the iron-oxidizer Mariprofundus ferrooxydans<\/em> PV-1. Conversely, the oxidized basaltic glass showed the absence of iron-oxides enriched surface deposits and correspondingly there was a lack of known iron-oxidizing bacteria in the inventoried diversity. In vitro<\/em>, a similar reduced basaltic glass was incubated in artificial seawater with a pure culture of the iron-oxidizing M. ferrooxydans<\/em> DIS-1 for 2 weeks, without any additional nutrients or minerals. Confocal Laser Scanning Microscopy revealed that the glass surface was covered by twisted stalks characteristic of this iron-oxidizing Zetaproteobacteria. This result supported findings of the in situ<\/em> experiments indicating that the Fe(II) present in the basalt was the energy source for the growth of representatives of Zetaproteobacteria in both the abyssal plain and the in vitro<\/em> experiment. In accordance, the surface alteration rind observed on the reduced basaltic glass incubated in situ<\/em> could at least partly result from their activity.<\/p>\n","protected":false},"template":"","tags":[],"publication_type":[19],"_links":{"self":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication\/7775"}],"collection":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/types\/publication"}],"wp:attachment":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/media?parent=7775"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/tags?post=7775"},{"taxonomy":"publication_type","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication_type?post=7775"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}