{"id":7689,"date":"2016-06-14T20:26:14","date_gmt":"2016-06-14T20:26:14","guid":{"rendered":"https:\/\/www.darkenergybiosphere.org\/?post_type=publication&#038;p=7677"},"modified":"2016-10-14T19:06:42","modified_gmt":"2016-10-14T19:06:42","slug":"deep-sea-hydrothermal-vent-epsilonproteobacteria-encode-a-conserved-and-widespread-nitrate-reduction-pathway-nap","status":"publish","type":"publication","link":"https:\/\/www.darkenergybiosphere.org\/publication\/deep-sea-hydrothermal-vent-epsilonproteobacteria-encode-a-conserved-and-widespread-nitrate-reduction-pathway-nap\/","title":{"rendered":"Deep-sea hydrothermal vent Epsilonproteobacteria encode a conserved and widespread nitrate reduction pathway (Nap)"},"content":{"rendered":"<p>Despite the frequent isolation of nitrate-respiring <i>Epsilonproteobacteria<\/i> from deep-sea hydrothermal vents, the genes coding for the nitrate reduction pathway in these organisms have not been investigated in depth. In this study we have shown that the gene cluster coding for the periplasmic nitrate reductase complex (<i>nap<\/i>) is highly conserved in chemolithoautotrophic, nitrate-reducing <i>Epsilonproteobacteria<\/i> from deep-sea hydrothermal vents. Furthermore, we have shown that the <i>napA<\/i> gene is expressed in pure cultures of vent <i>Epsilonproteobacteria<\/i> and it is highly conserved in microbial communities collected from deep-sea vents characterized by different temperature and redox regimes. The diversity of nitrate-reducing <i>Epsilonproteobacteria<\/i> was found to be higher in moderate temperature, diffuse flow vents than in high temperature black smokers or in low temperatures, substrate-associated communities. As NapA has a high affinity for nitrate compared with the membrane-bound enzyme, its occurrence in vent <i>Epsilonproteobacteria<\/i> may represent an adaptation of these organisms to the low nitrate concentrations typically found in vent fluids. Taken together, our findings indicate that nitrate reduction is widespread in vent <i>Epsilonproteobacteria<\/i> and provide insight on alternative energy metabolism in vent microorganisms. The occurrence of the <i>nap<\/i> cluster in vent, commensal and pathogenic <i>Epsilonproteobacteria<\/i> suggests that the ability of these bacteria to respire nitrate is important in habitats as different as the deep-sea vents and the human body.<\/p>\n","protected":false},"template":"","tags":[],"publication_type":[19],"_links":{"self":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication\/7689"}],"collection":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/types\/publication"}],"wp:attachment":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/media?parent=7689"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/tags?post=7689"},{"taxonomy":"publication_type","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication_type?post=7689"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}