{"id":16727,"date":"2021-07-16T08:22:27","date_gmt":"2021-07-16T15:22:27","guid":{"rendered":"https:\/\/www.darkenergybiosphere.org\/?post_type=publication&#038;p=16727"},"modified":"2021-08-31T22:02:11","modified_gmt":"2021-09-01T05:02:11","slug":"microbial-metabolism-and-adaptations-in-atribacteria%e2%80%90dominated-methane-hydrate-sediments","status":"publish","type":"publication","link":"https:\/\/www.darkenergybiosphere.org\/publication\/microbial-metabolism-and-adaptations-in-atribacteria%e2%80%90dominated-methane-hydrate-sediments\/","title":{"rendered":"Microbial metabolism and adaptations in Atribacteria\u2010dominated methane hydrate sediments"},"content":{"rendered":"<div class=\"abstract-group\">\n<section id=\"section-1-en\" class=\"article-section article-section__abstract\" lang=\"en\" data-lang=\"en\">\n<div class=\"article-section__content en main\">\n<p>Gas hydrates harbour gigatons of natural gas, yet their microbiomes remain understudied. We bioprospected 16S rRNA amplicons, metagenomes, and metaproteomes from methane hydrate-bearing sediments under Hydrate Ridge (offshore Oregon, USA, ODP Site 1244, 2\u201369\u2009mbsf) for novel microbial metabolic and biosynthetic potential.\u00a0<i>Atribacteria<\/i>\u00a0sequences generally increased in relative sequence abundance with increasing sediment depth. Most Atribacteria ASVs belonged to JS-1-Genus 1 and clustered with other sequences from gas hydrate-bearing sediments. We recovered 21 metagenome-assembled genomic bins spanning three geochemical zones in the sediment core: the sulfate\u2013methane transition zone, the metal (iron\/manganese) reduction zone, and the gas hydrate stability zone. We found evidence for bacterial fermentation as a source of acetate for aceticlastic methanogenesis and as a driver of iron reduction in the metal reduction zone. In multiple zones, we identified a Ni-Fe hydrogenase-Na<sup>+<\/sup>\/H<sup>+<\/sup>\u00a0antiporter supercomplex (Hun) in\u00a0<i>Atribacteria<\/i>\u00a0and\u00a0<i>Firmicutes<\/i>\u00a0bins and in other deep subsurface bacteria and cultured hyperthermophiles from the\u00a0<i>Thermotogae<\/i>\u00a0phylum.\u00a0<i>Atribacteria<\/i>\u00a0expressed tripartite ATP-independent transporters downstream from a novel regulator (AtiR). Atribacteria also possessed adaptations to survive extreme conditions (e.g. high salt brines, high pressure and cold temperatures) including the ability to synthesize the osmolyte di-myo-inositol-phosphate as well as expression of K<sup>+<\/sup>-stimulated pyrophosphatase and capsule proteins.<\/p>\n<\/div>\n<\/section>\n<\/div>\n","protected":false},"template":"","tags":[],"publication_type":[19],"_links":{"self":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication\/16727"}],"collection":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/types\/publication"}],"wp:attachment":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/media?parent=16727"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/tags?post=16727"},{"taxonomy":"publication_type","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication_type?post=16727"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}