{"id":16602,"date":"2021-04-23T12:41:08","date_gmt":"2021-04-23T19:41:08","guid":{"rendered":"https:\/\/www.darkenergybiosphere.org\/?post_type=publication&p=16602"},"modified":"2021-06-01T12:15:23","modified_gmt":"2021-06-01T19:15:23","slug":"geothermobacter-hydrogeniphilus-sp-nov-a-mesophilic-ironiii-reducing-bacterium-from-seafloor-subseafloor-environments-in-the-pacific-ocean-and-emended-description-of-the-genus-geothermobacter","status":"publish","type":"publication","link":"https:\/\/www.darkenergybiosphere.org\/publication\/geothermobacter-hydrogeniphilus-sp-nov-a-mesophilic-ironiii-reducing-bacterium-from-seafloor-subseafloor-environments-in-the-pacific-ocean-and-emended-description-of-the-genus-geothermobacter\/","title":{"rendered":"Geothermobacter hydrogeniphilus sp. nov., a mesophilic, iron(III)-reducing bacterium from seafloor\/subseafloor environments in the Pacific Ocean, and emended description of the genus Geothermobacter"},"content":{"rendered":"
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A novel mesophilic, anaerobic, mixotrophic bacterium, with designated strains EPR-MT<\/span>\u00a0and HR-1, was isolated from a semi-extinct hydrothermal vent at the East Pacific Rise and from an Fe-mat at L\u014d’ihi Seamount, respectively. The cells were Gram-negative, pleomorphic rods of about 2.0\u2009\u00b5m in length and 0.5\u2009\u00b5m in width. Strain EPR-MT<\/span>\u00a0grew between 25 and 45\u2009\u00b0C (optimum, 37.5\u201340\u2009\u00b0C), 10 and 50\u2009g l\u22121<\/span>\u00a0NaCl (optimum, 15\u201320\u2009g l\u22121<\/span>) and pH 5.5 and 8.6 (optimum, pH 6.4). Strain HR-1 grew between 20 and 45\u2009\u00b0C (optimum, 37.5\u201340\u2009\u00b0C), 10 and 50\u2009g l\u22121<\/span>\u00a0NaCl (optimum, 15\u201325\u2009g l\u22121<\/span>) and pH 5.5 and 8.6 (optimum, pH 6.4). Shortest generation times with H2<\/span>\u00a0as the primary electron donor, CO2<\/span>\u00a0as the carbon source and ferric citrate as terminal electron acceptor were 6.7 and 5.5\u2009h for EPR-MT<\/span>\u00a0and HR-1, respectively. Fe(OH)3<\/span>, MnO2<\/span>, AsO4<\/span>\u00a03-<\/span>, SO4<\/span>\u00a02-<\/span>, SeO4<\/span>\u00a02-<\/span>, S2<\/span>O3<\/span>\u00a02-<\/span>, S0<\/span>\u00a0and NO3<\/span>\u00a0–<\/span>\u00a0were also used as terminal electron acceptors. Acetate, yeast extract, formate, lactate, tryptone and Casamino acids also served as both electron donors and carbon sources. G+C content of the genomic DNA was 59.4\u200amol% for strain EPR-MT<\/span>\u00a0and 59.2\u200amol% for strain HR-1. Phylogenetic and phylogenomic analyses indicated that both strains were closely related to each other and to\u00a0Geothermobacter ehrlichii<\/a>\u00a0<\/span><\/span>, within the class \u03b4-\u00a0Proteobacteria<\/a>\u00a0<\/span><\/span>(now within the class\u00a0Desulfuromonadia<\/a>\u00a0<\/span><\/span>). Based on phylogenetic and phylogenomic analyses in addition to physiological and biochemical characteristics, both strains were found to represent a novel species within the genus\u00a0Geothermobacter<\/a>\u00a0<\/span><\/span>, for which the name\u00a0Geothermobacter hydrogeniphilus<\/span>\u00a0sp. nov. is proposed.\u00a0Geothermobacter hydrogeniphilus<\/span>\u00a0is represented by type strain EPR-MT<\/span>\u00a0(=JCM 32109T<\/span>=KCTC 15831T<\/span>=ATCC TSD-173T<\/span>) and strain HR-1 (=JCM 32110=KCTC 15832).<\/p>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"template":"","tags":[],"publication_type":[19],"_links":{"self":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication\/16602"}],"collection":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/types\/publication"}],"wp:attachment":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/media?parent=16602"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/tags?post=16602"},{"taxonomy":"publication_type","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication_type?post=16602"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}