{"id":14318,"date":"2020-01-06T12:15:40","date_gmt":"2020-01-06T20:15:40","guid":{"rendered":"https:\/\/www.darkenergybiosphere.org\/?post_type=publication&#038;p=14318"},"modified":"2020-01-06T12:15:41","modified_gmt":"2020-01-06T20:15:41","slug":"fine-tuned-protein-production-in-methanosarcina-acetivorans-c2a","status":"publish","type":"publication","link":"https:\/\/www.darkenergybiosphere.org\/publication\/fine-tuned-protein-production-in-methanosarcina-acetivorans-c2a\/","title":{"rendered":"Fine-Tuned Protein Production in Methanosarcina acetivorans C2A"},"content":{"rendered":"<p>Methanogenic archaea can be integrated into a sustainable, carbon-neutral cycle for producing organic chemicals from C<sub>1<\/sub>\u00a0compounds if the rate, yield, and titer of product synthesis can be improved using metabolic engineering. However, metabolic engineering techniques are limited in methanogens by insufficient methods for controlling cellular protein levels. We conducted a systematic approach to tune protein levels in\u00a0<i>Methanosarcina acetivorans<\/i>\u00a0C2A, a model methanogen, by regulating transcription and translation initiation. Rationally designed core promoter and ribosome binding site mutations in\u00a0<i>M.\u00a0acetivorans<\/i>\u00a0C2A resulted in a predicable change in protein levels over a 60 fold range. The overall range of protein levels was increased an additional 3 fold by introducing the 5\u2032 untranslated region of the\u00a0<i>mcrB<\/i>\u00a0transcript. This work demonstrates a wide range of precisely controlled protein levels in\u00a0<i>M.\u00a0acetivorans<\/i>\u00a0C2A, which will help facilitate systematic metabolic engineering efforts in methanogens.<\/p>\n","protected":false},"template":"","tags":[],"publication_type":[19],"_links":{"self":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication\/14318"}],"collection":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/types\/publication"}],"wp:attachment":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/media?parent=14318"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/tags?post=14318"},{"taxonomy":"publication_type","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication_type?post=14318"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}