{"id":12487,"date":"2018-03-26T13:53:13","date_gmt":"2018-03-26T20:53:13","guid":{"rendered":"https:\/\/www.darkenergybiosphere.org\/?post_type=publication&p=12487"},"modified":"2018-04-16T10:32:33","modified_gmt":"2018-04-16T17:32:33","slug":"thioclava-electrotropha-sp-nov-a-versatile-electrode-and-sulfur-oxidizing-bacterium-from-marine-sediments","status":"publish","type":"publication","link":"https:\/\/www.darkenergybiosphere.org\/publication\/thioclava-electrotropha-sp-nov-a-versatile-electrode-and-sulfur-oxidizing-bacterium-from-marine-sediments\/","title":{"rendered":"Thioclava electrotropha sp. nov., a versatile electrode and sulfur-oxidizing bacterium from marine sediments"},"content":{"rendered":"

A taxonomic and physiologic characterization was carried out on\u00a0Thioclava<\/a>\u00a0<\/span><\/em>strain ElOx9T<\/span>, which was isolated from a bacterial consortium enriched on electrodes poised at electron donating potentials. The isolate is Gram-negative, catalase-positive and oxidase-positive; the cells are motile short rods. The bacterium is facultatively anaerobic with the ability to utilize nitrate as an electron acceptor. Autotrophic growth with H2<\/sub>\u00a0and S0<\/span>\u00a0(oxidized to sulfate) was observed. The isolate also grows heterotrophically with organic acids and sugars. Growth was observed at salinities from 0 to 10% NaCl and at temperatures from 15 to 41\u2009\u00b0C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain belongs in the genus\u00a0Thioclava<\/a>\u00a0<\/span><\/em>; it had the highest sequence similarity of 98.8\u200a% to\u00a0Thioclava atlantica<\/a>\u00a0<\/span><\/em>13D2W-2T<\/span>, followed by\u00a0Thioclava dalianensis\u00a0<\/a><\/span><\/em>DLFJ1-1T<\/span>\u00a0with 98.5\u200a% similarity,\u00a0Thioclava pacifica<\/a>\u00a0<\/span><\/em>TL 2T<\/span>\u00a0with 97.7\u200a% similarity, and then\u00a0Thioclava indica<\/a>\u00a0<\/span><\/em>DT23-4T<\/span>\u00a0with 96.9\u200a%. All other sequence similarities were below 97\u200a% to characterized strains. The digital DNA\u2013DNA hybridization estimated when compared to\u00a0T. atlantica\u00a0<\/a><\/span><\/em>13D2W-2T<\/span>,\u00a0T. dalianensis<\/a>\u00a0<\/span><\/em>DLFJ1-1T<\/span>,\u00a0T. pacifica<\/a>\u00a0<\/span><\/em>TL 2T<\/span>\u00a0and\u00a0T. indica<\/a>\u00a0<\/span><\/em>DT23-4T<\/span>\u00a0were 15.8\u00b12.1, 16.7+2.1, 14.3\u00b11.9 and 18.3\u00b12.1\u200a%. The corresponding average nucleotide identity values between these strains were determined to be 65.1, 67.8, 68.4 and 64.4\u200a%, respectively. The G+C\u2009content of the chromosomal DNA is 63.4\u2009mol%. Based on these results, a novel species\u00a0Thioclava<\/a>\u00a0<\/span>electrotropha<\/em>\u00a0sp. nov. is proposed, with the type strain ElOx9T<\/span>\u00a0(=DSM 103712T<\/span>=ATCC TSD-100T<\/span>).<\/p>\n","protected":false},"template":"","tags":[],"publication_type":[19],"_links":{"self":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication\/12487"}],"collection":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/types\/publication"}],"wp:attachment":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/media?parent=12487"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/tags?post=12487"},{"taxonomy":"publication_type","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication_type?post=12487"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}