{"id":11602,"date":"2017-08-15T14:11:39","date_gmt":"2017-08-15T21:11:39","guid":{"rendered":"https:\/\/www.darkenergybiosphere.org\/?post_type=publication&#038;p=11602"},"modified":"2017-08-15T14:11:51","modified_gmt":"2017-08-15T21:11:51","slug":"sulfurovum-riftiae-sp-nov-a-mesophilic-thiosulfate-oxidizing-nitrate-reducing-chemolithoautotrophic-epsilonproteobacterium-isolated-from-the-tube-of-the-deep-sea-hydrothermal-vent-polychaete-rift","status":"publish","type":"publication","link":"https:\/\/www.darkenergybiosphere.org\/publication\/sulfurovum-riftiae-sp-nov-a-mesophilic-thiosulfate-oxidizing-nitrate-reducing-chemolithoautotrophic-epsilonproteobacterium-isolated-from-the-tube-of-the-deep-sea-hydrothermal-vent-polychaete-rift\/","title":{"rendered":"Sulfurovum riftiae sp. nov., a mesophilic, thiosulfate-oxidizing, nitrate-reducing chemolithoautotrophic epsilonproteobacterium isolated from the tube of the deep-sea hydrothermal vent polychaete Riftia pachyptila"},"content":{"rendered":"<p>An anaerobic, nitrate-reducing, sulfur- and thiosulfate-oxidizing bacterium, designated strain 1812E<span class=\"jp-sup\">T<\/span>, was isolated from the vent polychaete\u00a0<em class=\"\">Riftia pachyptila<\/em>, which was collected from a deep-sea hydrothermal vent on the East Pacific Rise. Cells were Gram-stain-negative rods, measuring approximately 1.05\u00b10.11 \u00b5m by 0.40\u00b10.05 \u00b5m. Strain 1812E<span class=\"jp-sup\">T\u00a0<\/span>grew at 25 \u2013 \u201345\u2009\u00b0C (optimum 35\u2009\u00b0C), with 1.5\u20134.0\u2009% (w\/v) NaCl (optimum 3.0\u2009%) and at pH 5.0\u20138.0 (optimum pH 6.0). The generation time under optimal conditions was 3\u2009h. Strain 1812E<span class=\"jp-sup\">T\u00a0<\/span>was an anaerobic chemolithotroph that grew with either sulfur or thiosulfate as the energy source and carbon dioxide as the sole carbon source. Nitrate was used as a sole terminal electron acceptor. The predominant fatty acids were C<sub class=\"\">16\u2009:\u20091<\/sub>\u00a0<em class=\"\">\u03c9<\/em>7<em class=\"\">c<\/em>, C<sub class=\"\">18\u2009:\u20091<\/sub>\u00a0<em class=\"\">\u03c9<\/em>7<em class=\"\">c<\/em>\u00a0and C<sub class=\"\">16\u2009:\u20090<\/sub>. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The major respiratory quinone was menaquinone MK-6 and the G+C content of the genomic DNA was 47.4 mol%. Phylogenetic analysis of the 16S rRNA gene of strain 1812E<span class=\"jp-sup\">T<\/span>\u00a0showed that the isolate belonged to the\u00a0<em class=\"\"><span class=\"named-content-class\"><a class=\"namesforlife\" title=\"Epsilonproteobacteria\" href=\"https:\/\/doi.org\/10.1601\/nm.3783\" target=\"xrefwindow\" rel=\"namesforlife-name\">Epsilonproteobacteria<\/a>\u00a0<\/span><\/em>, and its closest relatives were\u00a0<em class=\"\"><span class=\"named-content-species\"><a class=\"namesforlife\" title=\"Sulfurovum lithotrophicum\" href=\"https:\/\/doi.org\/10.1601\/nm.8874\" target=\"xrefwindow\" rel=\"namesforlife-name\">Sulfurovum lithotrophicum<\/a>\u00a0<\/span><\/em>42BKT<span class=\"jp-sup\">T\u00a0<\/span>and\u00a0<em class=\"\"><span class=\"named-content-species\"><a class=\"namesforlife\" title=\"Sulfurovum aggregans\" href=\"https:\/\/doi.org\/10.1601\/nm.25804\" target=\"xrefwindow\" rel=\"namesforlife-name\">Sulfurovum aggregans<\/a>\u00a0<\/span><\/em>Monchim 33<span class=\"jp-sup\">T\u00a0<\/span>(98.3 and 95.7\u2009% sequence similarity, respectively). DNA\u2013DNA relatedness between strain 1812E<span class=\"jp-sup\">T\u00a0<\/span>and the type strain of\u00a0<em class=\"\"><span class=\"named-content-species\"><a class=\"namesforlife\" title=\"S. lithotrophicum\" href=\"https:\/\/doi.org\/10.1601\/nm.8874\" target=\"xrefwindow\" rel=\"namesforlife-name\">S. lithotrophicum<\/a>\u00a0<\/span><\/em>was 29.7\u2009%, demonstrating that the two strains are not members of the same species. Based on the phylogenetic, molecular, chemotaxonomic and physiological evidence, strain 1812E<span class=\"jp-sup\">T<\/span>\u00a0represents a novel species within the genus\u00a0<em class=\"\"><span class=\"named-content-genus\"><a class=\"namesforlife\" title=\"Sulfurovum\" href=\"https:\/\/doi.org\/10.1601\/nm.8873\" target=\"xrefwindow\" rel=\"namesforlife-name\">Sulfurovum<\/a>\u00a0<\/span><\/em>, for which the name\u00a0<em class=\"\">Sulfurovum riftiae<\/em>\u00a0sp. nov. is proposed. The type strain is 1812E<span class=\"jp-sup\">T<\/span>\u00a0(=DSM 101780<span class=\"jp-sup\">T<\/span>=JCM 30810<span class=\"jp-sup\">T<\/span>).<\/p>\n","protected":false},"template":"","tags":[],"publication_type":[19],"_links":{"self":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication\/11602"}],"collection":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/types\/publication"}],"wp:attachment":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/media?parent=11602"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/tags?post=11602"},{"taxonomy":"publication_type","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication_type?post=11602"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}