{"id":11320,"date":"2017-06-01T11:14:17","date_gmt":"2017-06-01T18:14:17","guid":{"rendered":"https:\/\/www.darkenergybiosphere.org\/?post_type=publication&#038;p=11320"},"modified":"2017-06-15T11:48:37","modified_gmt":"2017-06-15T18:48:37","slug":"biogeochemical-n-signatures-from-rate-yield-trade-offs-during-in-vitro-chemosynthetic-no-3-reduction-by-deep-sea-vent-%ce%b5-proteobacteria-and-aquificae-growing-at-different-temperatures","status":"publish","type":"publication","link":"https:\/\/www.darkenergybiosphere.org\/publication\/biogeochemical-n-signatures-from-rate-yield-trade-offs-during-in-vitro-chemosynthetic-no-3-reduction-by-deep-sea-vent-%ce%b5-proteobacteria-and-aquificae-growing-at-different-temperatures\/","title":{"rendered":"Biogeochemical N signatures from rate-yield trade-offs during  in vitro  chemosynthetic NO 3 &#8211;  reduction by deep-sea vent  \u03b5-Proteobacteria  and  Aquificae  growing at different temperatures"},"content":{"rendered":"<p>NO<sub>3<\/sub><sup>&#8211;<\/sup> reduction is a metabolism that is widespread among <em>\u03b5-Proteobacteria<\/em> and <em>Aquificae<\/em>, two abundant classes of microorganisms found at deep-sea vents. In this study, we used <em>Sulfurovum lithotrophicum<\/em>, <em>Caminibacter mediatlanticus<\/em> and <em>Thermovibrio ammonificans<\/em> as representatives of these groups to study ecophysiological, metabolic and biogeochemical parameters associated with chemolithoautotrophic NO<sub>3<\/sub><sup>&#8211;<\/sup> reduction under different temperature regimes. We observed that while <em>S. lithotrophicum<\/em> and <em>C. mediatlanticus<\/em> achieved higher cell densities than <em>T. ammonificans<\/em>, the overall NO<sub>3<\/sub><sup>&#8211;<\/sup> consumption by the latter was on average \u223c 9 and \u223c 5 times faster on a per cell basis, respectively. Comparison with previously published data from other cultured vent <em>\u03b5-Proteobacteria<\/em> and <em>Aquificae<\/em> suggests that the rate-yield trade-offs observed in our experiments are generally conserved between these two groups in line with their ecophysiologies. Kinetic isotope effects of N from NO<sub>3<\/sub><sup>&#8211;<\/sup> reduction were 9.6 \u00b1 2.7 \u2030 for <em>S. lithotrophicum<\/em>, 6.4 \u00b1 0.7 \u2030 for <em>C. mediatlanticus<\/em> and 8.8 \u00b1 0.6 \u2030 for <em>T. ammonificans<\/em>. Our results help evaluate how metabolic partitioning between growth efficiency and reaction kinetics during chemolithoautotrophic NO<sub>3<\/sub><sup>&#8211;<\/sup> reduction affect the concentration and isotope composition of N compounds at deep-sea hydrothermal vents.<\/p>\n","protected":false},"template":"","tags":[],"publication_type":[19],"_links":{"self":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication\/11320"}],"collection":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/types\/publication"}],"wp:attachment":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/media?parent=11320"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/tags?post=11320"},{"taxonomy":"publication_type","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication_type?post=11320"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}