{"id":11083,"date":"2017-03-15T12:02:03","date_gmt":"2017-03-15T19:02:03","guid":{"rendered":"https:\/\/www.darkenergybiosphere.org\/?post_type=publication&#038;p=11083"},"modified":"2017-04-03T12:00:43","modified_gmt":"2017-04-03T19:00:43","slug":"a-new-solution-31p-nmr-sample-preparation-scheme-for-marine-sediments","status":"publish","type":"publication","link":"https:\/\/www.darkenergybiosphere.org\/publication\/a-new-solution-31p-nmr-sample-preparation-scheme-for-marine-sediments\/","title":{"rendered":"A new solution 31P NMR sample preparation scheme for marine sediments"},"content":{"rendered":"<p>A new approach for the preparation of marine sediment samples for solution <sup>31<\/sup>P nuclear magnetic resonance spectroscopy (<sup>31<\/sup>P NMR) has been developed and tested. This approach addresses important aspects associated with sample pretreatments for marine sediments, including the effects of sample pretreatment on sedimentary P composition. The method increases the signals of low abundance P species in <sup>31<\/sup>P NMR spectra by quantitatively and precisely removing up to 80% of inorganic P (orthophosphate) from sediment samples while causing minimal alteration of the chemical structure of organic P compounds. This method uses a reductive step to solubilize P bound to iron oxyhydroxides, followed by a low pH digestion to extract P from authigenic and biogenic apatite, as well as P bound to calcium carbonate. These P forms combined represent the most abundant inorganic P reservoir in marine sediments. The sample residue is then extracted in an alkaline solvent, 0.25 M NaOH with 0.05 M Na<sub>2<\/sub>EDTA, and processed for <sup>31<\/sup>P NMR spectroscopy. The method was tested on natural marine sediment samples from different localities with high inorganic P content (&gt;85% molybdate reactive P), and allowed for the detection of orthophosphate monoesters and pyrophosphate in samples for which only an orthophosphate signal could be resolved with an NaOH-EDTA extraction alone. This new approach will allow the use of <sup>31<\/sup>P NMR on samples for which low organic P concentrations previously hindered the use of this tool, and will help answer longstanding question regarding the fate of organic P in marine sediments.<\/p>\n","protected":false},"template":"","tags":[],"publication_type":[19],"_links":{"self":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication\/11083"}],"collection":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/types\/publication"}],"wp:attachment":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/media?parent=11083"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/tags?post=11083"},{"taxonomy":"publication_type","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication_type?post=11083"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}