{"id":10545,"date":"2017-01-13T10:45:34","date_gmt":"2017-01-13T18:45:34","guid":{"rendered":"https:\/\/www.darkenergybiosphere.org\/?post_type=publication&#038;p=10545"},"modified":"2017-02-01T12:22:02","modified_gmt":"2017-02-01T20:22:02","slug":"in-situ-incubation-of-iron-sulfur-mineral-reveals-a-diverse-chemolithoautotrophic-community-and-a-new-biogeochemical-role-for-thiomicrospira","status":"publish","type":"publication","link":"https:\/\/www.darkenergybiosphere.org\/publication\/in-situ-incubation-of-iron-sulfur-mineral-reveals-a-diverse-chemolithoautotrophic-community-and-a-new-biogeochemical-role-for-thiomicrospira\/","title":{"rendered":"In-situ incubation of iron-sulfur mineral reveals a diverse chemolithoautotrophic community and a new biogeochemical role for Thiomicrospira"},"content":{"rendered":"<p>Sulfide mineral precipitation occurs at mid\u2010ocean ridge (MOR) spreading centers, both in the form of plume particles and seafloor massive sulfide structures. A common constituent of MOR is the iron\u2010bearing sulfide mineral pyrrhotite, which was chosen as a substrate for <em>in\u2010situ<\/em> incubation studies in shallow waters of Catalina Island, CA to investigate the colonization of iron\u2010oxidizing bacteria. Microbial community datasets were obtained from <em>in\u2010situ<\/em> incubated pyrrhotite, allowing for direct comparison to microbial communities of iron\u2010sulfides from active and inactive chimneys in deep\u2010sea environments. Unclassified Gammaproteobacteria and Alphaproteobacteria (<em>Magnetovibrio)<\/em> largely dominated the bacterial community on pyrrhotite samples incubated in the water column while samples incubated at the surface sediment showed more even dominance by Deltaproteobacteria (<em>Desulfobulbus<\/em>), Gammaproteobacteria (Piscirickettsiaceae), Alphaproteobacteria (Rhodobacteraceae), and Bacteroidetes (Flavobacteriia). Cultivations that originated from pyrrhotite samples resulted in the enrichment of both, sheath\u2010forming and stalk\u2010forming Zetaproteobacteria. Additionally, a putative novel species of <em>Thiomicrospira<\/em> was isolated and shown to grow autotrophically with iron, indicating a new biogeochemical role for this ubiquitous microorganism.<\/p>\n","protected":false},"template":"","tags":[],"publication_type":[19],"_links":{"self":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication\/10545"}],"collection":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/types\/publication"}],"wp:attachment":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/media?parent=10545"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/tags?post=10545"},{"taxonomy":"publication_type","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication_type?post=10545"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}