{"id":10101,"date":"2016-11-15T21:00:30","date_gmt":"2016-11-15T21:00:30","guid":{"rendered":"https:\/\/www.darkenergybiosphere.org\/?post_type=publication&#038;p=10101"},"modified":"2016-11-15T21:00:31","modified_gmt":"2016-11-15T21:00:31","slug":"burkholderia-insulsa-sp-nov-a-facultatively-chemolithotrophic-bacterium-isolated-from-an-arsenic-rich-shallow-marine-hydrothermal-system","status":"publish","type":"publication","link":"https:\/\/www.darkenergybiosphere.org\/publication\/burkholderia-insulsa-sp-nov-a-facultatively-chemolithotrophic-bacterium-isolated-from-an-arsenic-rich-shallow-marine-hydrothermal-system\/","title":{"rendered":"Burkholderia insulsa sp. nov., a facultatively chemolithotrophic bacterium isolated from an arsenic-rich shallow marine hydrothermal system"},"content":{"rendered":"<p>Enrichment cultures inoculated with hydrothermally influenced nearshore sediment from Papua New Guinea led to the isolation of an arsenic-tolerant, acidophilic, facultatively aerobic bacterial strain designated PNG-April<span class=\"jp-sup\">T<\/span>. Cells of this strain were Gram-stain-negative, rod-shaped, motile and did not form spores. Strain PNG-April<span class=\"jp-sup\">T<\/span> grew at temperatures between 4 \u00b0C and 40 \u00b0C (optimum 30\u201337 \u00b0C), at pH 3.5 to 8.3 (optimum pH 5\u20136) and in the presence of up to 2.7\u200a% NaCl (optimum 0\u20131.0\u200a%). Both arsenate and arsenite were tolerated up to concentrations of at least 0.5 mM. Metabolism in strain PNG-April<span class=\"jp-sup\">T<\/span> was strictly respiratory. Heterotrophic growth occurred with O<span class=\"jp-sub\">2<\/span> or nitrate as electron acceptors, and aerobic lithoautotrophic growth was observed with thiosulfate or nitrite as electron donors. The novel isolate was capable of N<span class=\"jp-sub\">2<\/span>-fixation. The respiratory quinones were Q-8 and Q-7. Phylogenetically, strain PNG-April<span class=\"jp-sup\">T<\/span> belongs to the genus <span class=\"named-content-genus\"><a class=\"namesforlife namesforlife\" title=\"Burkholderia\" href=\"doi:10.1601\/nm.1619\" target=\"xrefwindow\" rel=\"namesforlife-name\"><span class=\"jp-italic\">Burkholderia<\/span> <\/a><\/span>and shares the highest 16S rRNA gene sequence similarity with the type strains of <span class=\"named-content-species\"><a class=\"namesforlife namesforlife\" title=\"Burkholderia fungorum\" href=\"doi:10.1601\/nm.1629\" target=\"xrefwindow\" rel=\"namesforlife-name\"><span class=\"jp-italic\">Burkholderia fungorum<\/span> <\/a><\/span>(99.8\u200a%), <span class=\"named-content-species\"><a class=\"namesforlife namesforlife\" title=\"Burkholderia phytofirmans\" href=\"doi:10.1601\/nm.9441\" target=\"xrefwindow\" rel=\"namesforlife-name\"><span class=\"jp-italic\">Burkholderia phytofirmans<\/span> <\/a><\/span>(98.8\u200a%), <span class=\"named-content-species\"><a class=\"namesforlife namesforlife\" title=\"Burkholderia caledonica\" href=\"doi:10.1601\/nm.1624\" target=\"xrefwindow\" rel=\"namesforlife-name\"><span class=\"jp-italic\">Burkholderia caledonica<\/span><\/a><\/span>(98.4\u200a%) and <span class=\"named-content-species\"><a class=\"namesforlife namesforlife\" title=\"Burkholderia sediminicola\" href=\"doi:10.1601\/nm.13257\" target=\"xrefwindow\" rel=\"namesforlife-name\"><span class=\"jp-italic\">Burkholderia sediminicola<\/span> <\/a><\/span>(98.4\u200a%). Differences from these related species in several physiological characteristics (lipid composition, carbohydrate utilization, enzyme profiles) and DNA\u2013DNA hybridization suggested the isolate represents a novel species of the genus <span class=\"named-content-genus\"><a class=\"namesforlife namesforlife\" title=\"Burkholderia\" href=\"doi:10.1601\/nm.1619\" target=\"xrefwindow\" rel=\"namesforlife-name\"><span class=\"jp-italic\">Burkholderia<\/span> <\/a><\/span>, for which we propose the name <span class=\"jp-italic\">Burkholderia insulsa<\/span> sp. nov. The type strain is PNG-April<span class=\"jp-sup\">T<\/span> (\u200a=\u200aDSM 28142<span class=\"jp-sup\">T<\/span>\u200a=\u200aLMG 28183<span class=\"jp-sup\">T<\/span>).<\/p>\n","protected":false},"template":"","tags":[],"publication_type":[19],"_links":{"self":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication\/10101"}],"collection":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/types\/publication"}],"wp:attachment":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/media?parent=10101"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/tags?post=10101"},{"taxonomy":"publication_type","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/publication_type?post=10101"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}