{"id":10401,"date":"2016-12-16T11:31:02","date_gmt":"2016-12-16T19:31:02","guid":{"rendered":"https:\/\/www.darkenergybiosphere.org\/?post_type=project_data&#038;p=10401"},"modified":"2017-02-01T16:02:20","modified_gmt":"2017-02-02T00:02:20","slug":"a-new-marine-sediment-sample-preparation-scheme-for-solution-31p-nmr-analysis","status":"publish","type":"data_project","link":"https:\/\/www.darkenergybiosphere.org\/data_project\/a-new-marine-sediment-sample-preparation-scheme-for-solution-31p-nmr-analysis\/","title":{"rendered":"A new marine sediment sample preparation scheme for  solution 31P NMR analysis"},"content":{"rendered":"<p>We developed and tested a new approach to\u00a0prepare\u00a0marine sediment samples for solution\u00a031P nuclear magnetic resonance spectroscopy (31P NMR). This approach addresses the effects of sample pretreatment on sedimentary P composition and increases the signal of low abundance P species in\u00a031P NMR spectra by removing up the majority inorganic P\u00a0 from sediment samples while causing minimal alteration of the chemical structure of organic P compounds. The method was tested on natural marine sediment samples from different localities (Equatorial Pacific, California\u00a0Margin\u00a0and\u00a0Arctic Ocean) with high inorganic P content, and allowed for the detection of low abundance P forms in samples for which only an orthophosphate signal could be resolved with\u00a0an NaOH-EDTA\u00a0extraction alone. This new approach will allow the use of\u00a031P NMR on samples for which low organic P concentrations previously hindered the use of this tool, and will help answer\u00a0longstanding\u00a0question regarding the fate of organic P in marine sediments.\u00a0We developed and tested a new approach to\u00a0prepare\u00a0marine sediment samples for solution 31P nuclear magnetic resonance spectroscopy (31P NMR). This approach addresses the effects of sample pretreatment on sedimentary P composition and increases the signal of low abundance P species in 31P NMR spectra by removing up the majority inorganic P\u00a0 from sediment samples while causing minimal alteration of the chemical structure of organic P compounds. The method was tested on natural marine sediment samples from different localities (Equatorial Pacific, California\u00a0Margin\u00a0and\u00a0Arctic Ocean) with high inorganic P content, and allowed for the detection of low abundance P forms in samples for which only an orthophosphate signal could be resolved with\u00a0an NaOH-EDTA\u00a0extraction alone. This new approach will allow the use of 31P NMR on samples for which low organic P concentrations previously hindered the use of this tool, and will help answer\u00a0longstanding\u00a0question regarding the fate of organic P in marine sediments.<\/p>\n","protected":false},"template":"","_links":{"self":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/data_projects\/10401"}],"collection":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/data_projects"}],"about":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/types\/data_project"}],"wp:attachment":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/media?parent=10401"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}