{"id":10031,"date":"2016-11-11T20:51:52","date_gmt":"2016-11-11T20:51:52","guid":{"rendered":"https:\/\/www.darkenergybiosphere.org\/?post_type=project_data&#038;p=10031"},"modified":"2017-07-10T13:46:37","modified_gmt":"2017-07-10T20:46:37","slug":"functional-potential-of-the-uncultivated-candidate-phylum-op9-from-deep-antarctic-marine-sediments-using-single-cell-genome-techniques","status":"publish","type":"data_project","link":"https:\/\/www.darkenergybiosphere.org\/data_project\/functional-potential-of-the-uncultivated-candidate-phylum-op9-from-deep-antarctic-marine-sediments-using-single-cell-genome-techniques\/","title":{"rendered":"Functional potential of the uncultivated Candidate Phylum OP9 from deep Antarctic marine sediments using single cell genome techniques"},"content":{"rendered":"<p>Bacteria belonging to the newly classified candidate phylum \u201cAtribacteria\u201d (formerly referred to as \u201cOP9\u201d and \u201cJS1\u201d) are common in anoxic methane-rich sediments. However, the metabolic functions and biogeochemical role of these microorganisms in the subsurface remains unrealized due to the lack of pure culture representatives. In this study of deep sediment from Antarctica\u2019s Ad\u00e9lie Basin, collected during Expedition 318 of the Integrated Ocean Drilling Program (IODP), Atribacteria-related sequences of the 16S rRNA gene were abundant (up to 51% of the sequences) and steadily increased in relative abundance with depth throughout the methane-rich zones. To better understand the metabolic potential of Atribacteria within this environment, and to compare with phylogenetically distinct Atribacteria from non-deep-sea environments, individual cells were sorted for single cell genomics from sediment collected from 97.41 m below the seafloor from IODP Hole U1357C. As observed for non-marine Atribacteria, a partial single cell genome suggests a heterotrophic metabolism, with Atribacteria potentially producing fermentation products such as acetate, ethanol, and CO2. These products may in turn support methanogens within the sediment microbial community and explain the frequent occurrence of Atribacteria in anoxic methane-rich sediments. This first report of a single cell genome from deep sediment broadens the known diversity within the Atribacteria phylum and highlights the potential role of Atribacteria in carbon cycling in deep sediment.<\/p>\n","protected":false},"template":"","_links":{"self":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/data_projects\/10031"}],"collection":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/data_projects"}],"about":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/types\/data_project"}],"wp:attachment":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/media?parent=10031"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}