{"id":391,"date":"2015-12-28T02:09:25","date_gmt":"2015-12-28T02:09:25","guid":{"rendered":"http:\/\/192.168.99.100:8080\/award\/functional-potential-of-the-uncultivated-candidate-phylum-op9-from-deep-antarctic-marine-sediments-using-single-cell-genome-techniques\/"},"modified":"2019-08-12T10:26:40","modified_gmt":"2019-08-12T17:26:40","slug":"functional-potential-of-the-uncultivated-candidate-phylum-op9-from-deep-antarctic-marine-sediments-using-single-cell-genome-techniques","status":"publish","type":"award","link":"https:\/\/www.darkenergybiosphere.org\/award\/functional-potential-of-the-uncultivated-candidate-phylum-op9-from-deep-antarctic-marine-sediments-using-single-cell-genome-techniques\/","title":{"rendered":"Functional potential of the uncultivated Candidate Phylum OP9 from deep Antarctic marine sediments using single cell genome techniques"},"content":{"rendered":"<p class=\"p1\">Bacteria belonging to the newly classified candidate phylum \u201cAtribacteria\u201d (formerly referred to as\u00a0&#8220;OP9&#8221; and \u201cJS1\u201d) are common in anoxic methane-rich sediments. However, the metabolic functions and\u00a0biogeochemical role of these microorganisms in the subsurface remains unrealized due to the lack of pure\u00a0culture representatives. In this study of deep sediment from Antarctica&#8217;s Ad\u00e9lie Basin, collected during\u00a0Expedition 318 of the Integrated Ocean Drilling Program (IODP), <em>Atribacteria<\/em>-related sequences of the\u00a016S rRNA gene were abundant (up to 51% of the sequences) and steadily increased in relative abundance\u00a0with depth throughout the methane-rich zones. To better understand the metabolic potential of\u00a0<em>Atribacteria<\/em> within this environment, and to compare with phylogenetically distinct <em>Atribacteria<\/em> from\u00a0non-deep-sea environments, individual cells were sorted for single cell genomics from sediment collected\u00a0from 97.41 meters below the seafloor from IODP Hole U1357C. As observed for non-marine\u00a0<em>Atribacteria<\/em>, a partial single cell genome suggests a heterotrophic metabolism, with <em>Atribacteria<\/em>\u00a0potentially producing fermentation products such as acetate, ethanol and CO<sub>2<\/sub>. These products may in turn\u00a0support methanogens within the sediment microbial community and explain the frequent occurrence of\u00a0<em>Atribacteria<\/em> in anoxic methane-rich sediments. This first report of a single cell genome from deep\u00a0sediment broadens the known diversity within the <em>Atribacteria<\/em> phylum and highlights the potential role of\u00a0<em>Atribacteria<\/em> in carbon cycling in deep sediment.<\/p>\n","protected":false},"template":"","meta":[],"award_type":[10],"_links":{"self":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/awards\/391"}],"collection":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/awards"}],"about":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/types\/award"}],"wp:attachment":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/media?parent=391"}],"wp:term":[{"taxonomy":"award_type","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/award_type?post=391"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}