{"id":303,"date":"2015-12-28T02:09:22","date_gmt":"2015-12-28T02:09:22","guid":{"rendered":"http:\/\/192.168.99.100:8080\/award\/microbial-activity-in-oxygenated-subseafloor-sediment\/"},"modified":"2019-08-12T10:29:46","modified_gmt":"2019-08-12T17:29:46","slug":"microbial-activity-in-oxygenated-subseafloor-sediment","status":"publish","type":"award","link":"https:\/\/www.darkenergybiosphere.org\/award\/microbial-activity-in-oxygenated-subseafloor-sediment\/","title":{"rendered":"Microbial activity in oxygenated subseafloor sediment"},"content":{"rendered":"<p>The purpose of this project was to apply metatranscriptomic methods to North Atlantic pelagic clay sediment, in order to better understand the microbial activities in this habitat.\u00a0 Deep-sea pelagic clays contain extremely low organic matter and oxygen penetrates deeply into the sediment, which should be the major high-energy electron acceptor supporting microbial life.\u00a0 However, cell counts in pelagic clays are relatively low suggesting they are limited by availability of electron donors (e.g., organic matter).\u00a0 In contrast, organic rich yet oxidant limited continental margin sediment has much higher cell abundances.\u00a0 This project has produced new metatranscriptomes in electron donor limited pelagic clays from two sites in the North Atlantic, which are being compared to existing metatranscriptomic data from oxidant limited Peru Margin sediment.\u00a0 Metatranscriptomes from one hole have been sequenced and preliminary analysis indicates active DNA repair, energy production, and organic matter degradation by the <i>in situ<\/i> microbial communities.\u00a0 These data are currently being analyzed in greater detail, and funds are being sought to sequence the metatranscriptome libraries from a second hole, which will strengthen the conclusions. The results will be compared to metatranscriptomes from oxidant limited Peru Margin, in order to better understand subseafloor microbial adaptations and activities under electron donor versus oxidant limitation.<\/p>\n","protected":false},"template":"","meta":[],"award_type":[11],"_links":{"self":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/awards\/303"}],"collection":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/awards"}],"about":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/types\/award"}],"wp:attachment":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/media?parent=303"}],"wp:term":[{"taxonomy":"award_type","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/award_type?post=303"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}