{"id":177,"date":"2015-12-28T02:09:17","date_gmt":"2015-12-28T02:09:17","guid":{"rendered":"http:\/\/192.168.99.100:8080\/award\/combining-omics-approaches-to-gain-a-comprehensive-understanding-of-microbial-diversity-and-activity-in-subsurface-igneous-basement-along-the-louisville-seamount-trail-iodp-expedition-330\/"},"modified":"2019-08-12T10:33:14","modified_gmt":"2019-08-12T17:33:14","slug":"combining-omics-approaches-to-gain-a-comprehensive-understanding-of-microbial-diversity-and-activity-in-subsurface-igneous-basement-along-the-louisville-seamount-trail-iodp-expedition-330","status":"publish","type":"award","link":"https:\/\/www.darkenergybiosphere.org\/award\/combining-omics-approaches-to-gain-a-comprehensive-understanding-of-microbial-diversity-and-activity-in-subsurface-igneous-basement-along-the-louisville-seamount-trail-iodp-expedition-330\/","title":{"rendered":"Combining omics approaches to gain a comprehensive understanding of microbial diversity and activity in subsurface igneous basement along the Louisville Seamount Trail (IODP Expedition 330)"},"content":{"rendered":"<p>This project aimed to increase our understanding of the diversity, genomic potential, and activity of microbes living in three samples from deep subsurface oceanic crust located at Integrated Ocean Drilling Program (IODP) Site U1374, Rigil Guyot of the Louisville Seamount Chain.\u00a0 We sought to integrate three approaches to analyzing microbial diversity by sequencing whole community DNA, genomic DNA from single sorted cells, and actively transcribed genes from the whole community.\u00a0 The integrated analysis of these three datasets from the same sample provides a complimentary picture of subsurface microbiology whose net worth is far greater than the sum of its parts.\u00a0 Attempts to sort single cells from subsurface volcanic basement proved to be too difficult, however, and ultimately failed after multiple attempts.\u00a0 Despite this, we advanced understanding of how to approach single cell genomics with basement samples, and were able to photograph particles sorted with a flow cytometer, which revealed the apparent presence of spores and non-biogenic material.\u00a0 Given the difficulties encountered with the basement samples, we switched our focus to use the same methodology with microbial mats that form at diffuse venting sites along the Mid-Atlantic Ridge.\u00a0 Samples from Rainbow and TAG hydrothermal fields were sorted and 30 single cell genomes and one isolate genome were analyzed, targeting <em>Zetaproteobacteria<\/em> and understudied genera common to hydrothermal microbial mats, including 3 GNO2, 5 <em>Chlorobi<\/em>, 2 <em>Methylococcales<\/em> and 6 <em>Gammaproteobacteria<\/em> believed to be methylotrophs.\u00a0 In parallel, a metagenome and metatranscriptome was analyzed from the same TAG sample as the single cell genomes, as well as three other MAR metagenomes and one paired metatranscriptome.\u00a0 Analysis is underway and will target microbial understanding food web interactions in these subsurface derived mats.<\/p>\n","protected":false},"template":"","meta":[],"award_type":[11],"_links":{"self":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/awards\/177"}],"collection":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/awards"}],"about":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/types\/award"}],"wp:attachment":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/media?parent=177"}],"wp:term":[{"taxonomy":"award_type","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/award_type?post=177"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}