{"id":175,"date":"2015-12-28T02:09:17","date_gmt":"2015-12-28T02:09:17","guid":{"rendered":"http:\/\/192.168.99.100:8080\/award\/a-new-tool-for-rapidly-screening-single-cells-from-deepsea-subsurface-environments-for-genomic-identification\/"},"modified":"2018-03-27T12:37:07","modified_gmt":"2018-03-27T19:37:07","slug":"a-new-tool-for-rapidly-screening-single-cells-from-deepsea-subsurface-environments-for-genomic-identification","status":"publish","type":"award","link":"https:\/\/www.darkenergybiosphere.org\/award\/a-new-tool-for-rapidly-screening-single-cells-from-deepsea-subsurface-environments-for-genomic-identification\/","title":{"rendered":"A new tool for rapidly screening single cells from deepsea subsurface environments for genomic identification"},"content":{"rendered":"<p>A current limitation for biologically meaningful genomic analysis of single cells obtained by sorting from deep marine subsurface sediments<br \/>\nis the absence of a rapid, cost-\u00ad\u2010effective molecular tool to help decide, which cells to advance for single cell genome sequencing. We<br \/>\nimplemented and tested on subsurface sediments-\u00ad\u2010derived single cells a unique nanoLiter qPCR tool that we have recently developed for<br \/>\napplication to study population biology in complex environments. This nL-\u00ad\u2010qPCR tool can interrogate simultaneously in up to several hundred<br \/>\ndifferent qPCR reactions in nL samples the presence and abundance of candidate genes, and therefore provide a high level phylogenetic and<br \/>\ngenomic overview of the genomic content of a cell. We showed that this information is key when screening hundreds of sorted single cells for<br \/>\nmaking decisions which amplified genomes to subject to sequencing. We developed and tested this new tool on the group of Chloroflexi, which<br \/>\nare present and sometimes dominant at numerous subsurface sites and used it to identify subpopulations for single cell genome sequencing.<\/p>\n","protected":false},"template":"","meta":[],"award_type":[11],"_links":{"self":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/awards\/175"}],"collection":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/awards"}],"about":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/types\/award"}],"wp:attachment":[{"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/media?parent=175"}],"wp:term":[{"taxonomy":"award_type","embeddable":true,"href":"https:\/\/www.darkenergybiosphere.org\/wp-json\/wp\/v2\/award_type?post=175"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}