Building on the enthusiasm of our first virtual annual meeting in 2020, we continue to bring the C-DEBI community together through a virtual meeting series. These virtual events are held monthly during the first week of each month, with a regular rotation of science workshops and professional development workshops. Each workshop contains about 90 minutes of programming, including plenary presentations by invited speakers, a community building breakout session, and a plenary Q&A session. To foster an open environment for data sharing and discussion, sessions will not be recorded.
The layout of the 90-minute meetings (all 10:30AM – 12:00PM Pacific Time) is anticipated to be:
- Welcome (10 minutes)
- Community building ice breaker (10 minutes; in breakout rooms if the group is large)
- Plenary talk(s) (30 minutes)
- Open Q&A (30 minutes)
- Wrap up (10 minutes)
Friday July 9, 2021 (10:30AM – 12:00PM Pacific Time)
Techniques for Isolating DNA from Tough Samples [Register for connection information]
|Jackie Goordial, University of Guelph
Squeezing DNA from a rock: DNA extractions from low biomass settings
In this tutorial I will draw on experience in mineral permafrost and ocean crust samples to discuss methods to obtain genomic material from low biomass samples. I will discuss how (and when) to tweak common commercial DNA kits for low biomass samples (including lysis considerations and elution alterations), the use of flow cytometry to concentrate cells, and finally – options for amplifying DNA for downstream analysis when you get very small amounts.
Tentatively: Friday August 6, 2021 (10:30AM – 12:00PM Pacific Time)
Techniques for Isolating Persnickety Microbes
|Roman Barco, University of Southern California|
|Ileana Pérez-Rodríguez, University of Pennsylvania
From microbes in nature to nature in microbes
Deliberate cultivation and isolation of microorganisms associated with rock-water reactions in oceanic crust provides direct understanding of microbial physiologies and metabolisms, and how they are expressed under controlled and measurable physicochemical conditions. Specifically, I will show how my cultivation studies with anaerobic hydrogenotrophs from seafloor and subseafloor environments inform understanding of microbial speciation and microbial community structure in hydrothermal ecosystems. Studying microbial responses to environmental parameters also advances their utility for biotechnological applications.
If you are an early career researcher (student, postdoc, junior faculty, etc.) who would like to help coordinate these events, please contact us!
Joy Buongiorno, Maryville College
James Bradley, Queen Mary University of London
Beth Orcutt, C-DEBI Senior Scientist, Bigelow Laboratory for Ocean Sciences
Rosalynn Sylvan, C-DEBI Managing Director, University of Southern California