URLhttps://www.bco-dmo.org/dataset/653698
Download URLhttps://www.bco-dmo.org/dataset/653698/data/download
Media Typetext/tab-separated-values
CreatedAugust 10, 2016
ModifiedSeptember 9, 2016
StateFinal no updates expected
Brief DescriptionSequence accession numbers for archaeal and bacterial clone libraries from Guaymas Basin sediments

Acquisition Description

Hydrothermal sediments were sampled using HOV Alvin.

For complete acquisition and processing details, refer to:
McKay, L., et al. 2016. Thermal and geochemical influences on microbial biogeography in the hydrothermal sediments of Guaymas Basin, Gulf of California. Environmental Microbiology Reports, 8(1), 150-161. doi:10.1111/1758-2229.12365

Processing Description

For complete acquisition and processing details, refer to:
McKay, L., et al. 2016. Thermal and geochemical influences on microbial biogeography in the hydrothermal sediments of Guaymas Basin, Gulf of California. Environmental Microbiology Reports, 8(1), 150-161. doi:10.1111/1758-2229.12365

Parameters

sequence_type [sample_descrip]
Details
sequence_type
Description of the type of sequence
text description of sample collected
sample_location [site_descrip]
Details
sample_location
Location where sample was collected

Description or characteristics of a sampling site

Details
method
Sequencing method used
Method used to collect sample, for example Bongo net or Methot trawl.
accession_num [accession number]
Details
accession_num
NCBI accession number
Database identifier assigned by repository and linked to GenBank or other repository.
popset_num [accession number]
Details
popset_num
NCBI Popset number
Database identifier assigned by repository and linked to GenBank or other repository.
closely_related_to [unknown]
Details
closely_related_to
Description of closely related taxa
association with a community-wide standard parameter is not yet defined
accession_link [accession number]
Details
accession_link
Hyperlink to NCBI for the accession number
Database identifier assigned by repository and linked to GenBank or other repository.
popset_link [accession number]
Details
popset_link
Hyperlink to NCBI for the Popset
Database identifier assigned by repository and linked to GenBank or other repository.

Dataset Maintainers

NameAffiliationContact
Andreas P. TeskeUniversity of North Carolina at Chapel Hill (UNC-Chapel Hill)
Luke J. McKayUniversity of Montana
Shannon RauchWoods Hole Oceanographic Institution (WHOI BCO-DMO)

BCO-DMO Project Info

Project TitleConstraints on microbial biogeography in hydrothermally active sediments of Guaymas Basin: Energetic limits, physical stressors, and upward compression of metabolic zones
AcronymMicrobial Biogeo Guaymas
URLhttps://www.bco-dmo.org/project/637539
CreatedJanuary 29, 2016
ModifiedJanuary 29, 2016
Project Description

Project description from C-DEBI:
Shallow subsurface temperatures can reach extreme levels in just 40 cm depth in Guaymas Basin sediments, limiting microbial colonization to thermally tolerable surface sediments. At temperatures beyond approximately 80 degrees C and 100 degrees C, respectively, the 13C-isotopic signatures of microbial anaerobic oxidation of methane and organic matter remineralization appear to be thermally restricted. Putative methane consuming archaea dominate the archaeal clone library while sulfur cycling bacteria andChloroflexi-related sequences dominate the bacterial clone library. Archaeal clone library data suggest that the ANME-1 Guaymas archaea tolerate high in situ temperatures up to approximately 80 degrees C, thereby gaining an advantage in access to the geothermal methane pool in hot Guaymas Basin sediments. Lastly, the results indicate that in situ thermal and/or geochemical gradients structure archaeal community composition and biogeography more than bacterial biogeography.

While the average upper thermal temperature for detectable microbial life by RNA recovery in Guaymas Basin sediments appears to be around 80 degrees C, temperatures may fluctuate by 25 degrees C in as little as a day. Isotopic evidence for microbially mediated methane oxidation is only slight, yet putative methanotrophic archaea are commonly recovered in nearly all samples suggesting they may perform other physiological modes or isotopic signatures are not detectable because of high methane concentrations. High temperature associated archaea appear to be represented by OTUs related to uncultured MCG and ANME-1 Guaymas groups. For bacteria the dominant high temperature associated OTU was phylogenetically associated with the Thermodesulfobacteriaceae.

Two of the four main themes of C-DEBI research are "Extent of Life" and "Limits of Life". Using sediment samples acquired from Guaymas Basin, my C-DEBI research links these two themes by examining how the biogeographical distribution of sedimentary microorganisms is shaped by severe, life-limiting conditions. Although these samples are not from deep sediments, they exemplify deep biogeochemical processes that have been compressed to shallower depths by elevated hydrothermal activity. My research demonstrates how thermal and geochemical regimes interact to control the spatial extent of life by focusing on microbial zonation in an energetically diverse hydrothermal environment. My intention with this research was to accurately describe microbial biogeography and the physicochemical factors controlling it in these unique, compressed sediments, which can be a useful asset in preparation for future IODP sampling procedures and analyses as well as investigations in deep subsurface microbiology around the world.

Project Maintainers
NameAffiliationRoleContact
Luke J. McKayMontana State UniversityPrincipal Investigator
Andreas P. TeskeUniversity of North Carolina at Chapel Hill (UNC-Chapel Hill)Co-Principal Investigator
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