Project Title Uncovering novel mechanisms of extracellular electron uptake in subsurface-relevant marine bacterial isolates
Acronym bacterial isolates electron uptake
Created April 13, 2020
Modified April 13, 2020

Project Description

Abstract (from C-DEBI, for subaward USC 104889896.

Though the Earth’s subsurface supports the largest reservoir of biomass on the planet, there are major questions as to how life in these environments persists, and/or what energy sources and sinks are utilized.  Due to the dearth of knowledge surrounding lithotrophic or “rock eating” metabolisms, especially those that utilize solid phase minerals and/or are cryptic in nature, the potential role of these substrates as electron sources in many habitats remains unclear. This proposal seeks to improve our understanding of mineral-oxidation processes in a range of subsurface-relevant taxa. This work will utilize comparative genomics along with high throughput genetic screens to highlight genes involved in mineral oxidation in eight marine sediment microbes with unique electron uptake capabilities.  These organisms are closely related to strains isolated from a wide range of marine sediments, and subsurface habitats. The outcomes of this work will, not only further our understanding of lithotrophic metabolisms, but the functional gene information gleaned will aid in the detection of microbe-mineral interactions using bioinformatic approaches. This work has the potential to enhance scientific participation in individuals underrepresented in STEM fields by funding an early career scientist, and aiding in the training of an undergraduate researcher.

Data Project Maintainers

Annette R. RoweUniversity of Cincinnati (UC)Principal Investigator