Chemolithoautotrophic iron oxidizers play an important role in biogeochemical cycling in deep ocean biosphere. Zetaproteobacteria are widely distributed in deep ocean biosphere and are known to be obligately dependent on Fe(II) oxidation to fix carbon dioxide to grow. Thus Zetaproteobacteria play a potentially significant role in biogeochemical iron cycling in the deep ocean biosphere. To elucidate and quantify their contribution to iron cycling and microbial communities, it is important to understand the genes involved and mechanisms of iron oxidation. This objective will require a genetic system to perform genetic manipulation in Zetaproteobacteria. Currently there are no genetic systems available owing to the low growth yield, accumulation of mineral oxides during growth and their inability to make colonies on solidified medium, a prerequisite for classical genetic techniques. I propose to develop a genetic system in Zetaproteobacteria using Mariprofundus ferrooxydans PV-1 as a model organism. Using this genetic system, a new metabolism will be provided to domesticate this strain to make colonies on solidified medium to further study iron oxidation mechanism. I propose to participate as an instructor for microbial diversity to incoming students. Furthermore, I will mentor undergraduate students to follow a research career in microbiology.