Our understanding of microbial life within the seafloor of the dark ocean is still in its infancy; particularly, with respect to the largely inaccessible sediment-covered ocean crust. Despite our profound lack of access, some experts argue that the upper ocean basement is among the most suitable subseafloor environment for microbial life. During my tenure as a C-DEBI Graduate Fellowship, I explored the deep biosphere of the subseafloor crust on the eastern flank of the Juan de Fuca Ridge by retrieving pristine crustal fluids via seafloor sampling and instrumentation platforms. Analysis of the microbial life was highly successful and resulted in several publications including one that describes a novel microbial assemblage within the basement fluid environment that is distinct from sediment and seawater, and another that reveals temporally dynamic microbial communities in the deep subsurface. Investigations are ongoing using metagenomic and metatranscriptomic approaches and, though in the preliminary stages, indicate that sulfate-reduction, methanogenesis, and fermentation are popular themes in the anoxic basaltic biosphere. The phylogenetic and implied physiological diversity in the oceanic crust is of broad interest due to the contribution to global biomass, elemental cycling, and astrobiology topics related to subsurface chemosynthetic-based ecosystems. The C-DEBI Graduate Fellowship allowed me to pursue this exciting work and facilitated many opportunities that helped to establish my professional scientific network.