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Awards > Research Exchange Grants
Phosphorus uptake mechanisms in the deep sedimentary biosphere
PI: Delphine Defforey (University of California, Santa Cruz)
Current Placement: Associate Editor, Nature Communications
Advisor: Adina Paytan (University of California, Santa Cruz)
Host: Brandi Kiel Reese (Texas A&M University, Corpus Christi)
Amount: $3,000.00
Award Dates: March 23, 2015 — April 6, 2015

Abstract

The goal of my research exchange at Dr. Brandi Kiel Reese’s laboratory was to extract DNA from two sediment samples from North Pond (IODP Expedition 336, Hole U1382B) for metagenomic analyses. This project aims to understand how the deep biosphere obtains phosphorus (P) and impacts sedimentary P cycling by determining the presence or absence of key genes involved in microbial P uptake and metabolism using metagenomic analyses. The proposed work is directly related to C-DEBI central research themes and will greatly enhance our knowledge of the activity in the deep biosphere and the limits of life. Our results will be compared to P geochemical data from North Pond (IODP expedition 336), which involve sequential extractions, stable oxygen isotopes in phosphate (proxy for P cycling) and 31P nuclear magnetic resonance spectroscopy (31P NMR) data. The combined use of molecular microbial ecology techniques and geochemistry will provide a unique perspective on P sources to the deep biosphere and potential impacts of the deep biosphere on P cycling.

Related Items

Publications
Publications > Proceedings
Published: September 2, 2015
Proceedings of the IODP
Data report: characteristics of sedimentary phosphorus at North Pond, IODP Expedition 336
Authors: Delphine Defforey, Adina Paytan
C-DEBI Contribution Number: 256
Publications > Journal Article
Published: February 1, 2017
Limnology and Oceanography: Methods
A new solution 31P NMR sample preparation scheme for marine sediments
Authors: Delphine Defforey, Barbara J. Cade-Menun, Adina Paytan
C-DEBI Contribution Number: 353
Project Data
Project Data
Last Modified: November 7, 2016
A new marine sediment sample preparation scheme for solution 31P NMR analysis
Project Maintainers: Adina Paytan, Delphine Defforey, Barbara J. Cade-Menun
Project Data
Last Modified: November 7, 2016
Phosphorus cycling in ridge-flank sediments
Project Maintainers: Adina Paytan, Delphine Defforey
Project Data
Last Modified: November 7, 2016
Potential phosphorus uptake mechanisms of the deep sedimentary biosphere
Project Maintainers: Adina Paytan, Benjamin J. Tully, Jason B. Sylvan, Delphine Defforey, Barbara J. Cade-Menun, Brandi Kiel Reese, Laura A. Zinke

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