The goals of this project are to comprehensively describe the potential metabolic attributes, evolutionary history, and population genetic/microevolutionary characteristics of members of the bacterial phylum Nitrospirae inhabiting the basalt-hosted deep subseafloor of the Juan de Fuca Ridge flank in the Northeast Pacific Ocean. This will be accomplished by leveraging an existing suite of crustal fluid samples from research cruises spanning 2008-2014 (including existing geochemical and physical metadata), and high throughput DNA sequencing from metagenomes and genome sequences generated from single microbial cells by the Department of Energy’s Joint Genome Institute through a Community Sequencing Program Award.
