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[PDF
Agenda]
 Andreas
Teske's tour at the Old Well |
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 The historic Carolina Inn |
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 Laboratory
training exercises |
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 Tom McCollom rocks C-DEBI! |
| Deep Sediment Microbiology Now |
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Welcome to DEBI, the RCN, and the
Deep Sediment Biosphere workshop
Andreas
Teske |
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Deep sediment microbiology and ODP:
An overview [PDF]
Andreas
Teske |
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Energy constraints of microbial
growth and survival
[PDF]
Tori
Hoehler |
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'North Pond' and 'South Pacific
Gyre' updates; future directions
[no PDF due to proprietary content]
Wiebke
Ziebis |
| Breakout Session I: Fundamental
Microbiology Questions about the Deep Biosphere |
Breakout session IA: Genomics, evolution and biogeography
Leaders: Eric
Boyd, Billy
Brazelton, Karla
Heidelberg
Discussion of the nature of microbial ecology in the deep
sediment subsurface, the implications for microbial genomes,
genome evolution, and microbial distributions.
Breakout session IB: Microbial activity, physiology,
metabolism
Leaders: Karine
Alain, Suzanna
Bräuer, Alberto
Robador, Daniel
Rogers
Discussion of the biological adaptations that may be specific
to the deep subsurface in terms of energy utilization, growth,
and survival.
| What to do Better the Next
Time |
Sediment-related potential projects and ideas
Participant mini-presentations: Karine
Alain/Maria
Ciobanu, Jen
Biddle, Brandon
Briggs, Gaëtan
Burgaud, Ginny
Edgcomb [PDF],
Jiasong
Fang, Hector
Hernandez, Heath
Mills
Posters were available for viewing during the three hour
poster session over dinner. [More]
| Technology Challenges and
Innovations |
Detecting and probing subseafloor life: New technological
challenges
Jens
Kallmeyer, Yuki
Morono, Axel
Schippers
Quantification of Bacteria and Archaea in deeply
buried marine sediments [PDF]
Axel
Schippers
Contamination control, nucleic acid isolation, amplification,
and quantification [PDF]
Mark
Lever, Karen
Lloyd
Technological advances in cultivating microbes and
microbial activity measurement
Mandy Joye,
Karsten Zengler
| Samples, Ships, and the System |
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Opportunities with DEBI and C-DEBI
Jan
Amend, Rosalynn
Lee |
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Upcoming IODP cruises and how to
get involved: Beyond IODP and after 2013 [PDF]
Geoff
Wheat |
| Breakout Session II: New
Strategies and Technical Innovation |
Breakout session IIA: Assessing physiological adaptations
and energy constraints – research priorities or the
deep sedimentary biosphere
Leaders: Tom
McCollom, Roy
Price, Karyn
Rogers, Everett
Shock
Discussion of the current state of knowledge on tools available
to study microbiology of the deep subsurface, their limitations,
what new tools might be employed, and what needs to be developed.
Breakout session IIB: Sensitive detection and identification
of microbial diversity and function: Problems and solutions
Leaders:
Ginny
Edgcomb, D’Arcy
Meyer-Dombard
Discussion of the current technologies used in characterizing
the microbiology of deep subsurface environments, what they
tell us, what they don’t, and where we need to focus
our efforts.
These activities ran in three major sessions: Microscopy,
Cultivation, Sequence Analysis. Each block was taught three
times, so students could alternate. The instructors did a
heroic job and tagged-team as they saw fit.
- Exercises in Microscopy - Microscopy
in sediments; single cells and substrate-attached biofilms;
automated methods. Jens
Kallmeyer, Axel
Schippers, Matt
Schrenk, Olympus representatives
- Exercises in Cultivation: Novel cultivation
techniques; growth and activity at high pressure. Mandy
Joye/Marshall
Bowles, Karsten
Zengler
- Exercises in Sequence Analysis: Pyrosequencing
data analysis, functional genes, and guide to software for
sequence data. Jennifer
Biddle, Mark
Lever, Karen
Lloyd
[More]
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Workshop recap
[PDF]
Andreas
Teske |
> See the workshop
summary report.
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